Review



macrophage colony  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    MedChemExpress macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/M-CSF%2C+Mouse/pmc13471083-120-17-23
    Average 98 stars, based on 35 article reviews
    macrophage colony - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling"

    Article Title: APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1902143

    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.
    Figure Legend Snippet: Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Techniques Used: Isolation, Staining, Flow Cytometry, Fluorescence, Two Tailed Test, Derivative Assay

    Related Articles

    Generated:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: CD14 + monocytes were isolated and differentiated in RPMI-1640 medium (MeilunBio, MA0215) containing 10% FBS (MeilunBio, PWL217) and human M-CSF (MCE, HY-P7050) for 7 days. .. Mouse bone marrow–derived macrophages (BMDMs) were generated from C57BL/6 mice (6–8 weeks old) by culturing bone marrow cells in high-glucose DMEM (MeilunBio, MA0212) supplemented with 10% FBS (MeilunBio, PWL217) and mouse M-CSF (MCE, HY-P7085) for 7 days. ..

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: CD14+ monocytes were isolated and differentiated in RPMI-1640 medium (MeilunBio, MA0215) containing 10% FBS (MeilunBio, PWL217) and human M-CSF (MCE, HYP7050) for 7 days. .. Mouse bone marrow–derived macrophages (BMDMs) were generated from C57BL/6 mice (6–8 weeks old) by culturing bone marrow cells in high-glucose DMEM (MeilunBio, MA0212) supplemented with 10% FBS (MeilunBio, PWL217) and mouse M-CSF (MCE, HY-P7085) for 7 days. ..

    Magnetic Beads:

    Article Title: PRC1 promotes immunosuppressive macrophages in sepsis via β-catenin/STAT3 signaling
    Article Snippet: MSAB (Cat#S6901) and SKL2001 (Cat#S8320) were purchased from Selleck Chemicals (TX, USA). .. Protein A/G Magnetic Beads (Cat#HY-K0202), Mouse M-CSF (CatHY-P7085), human IFN-γ (Cat#HY-P7025) and mouse IFN-γ (Cat#HY-P7071) were purchased from MedChemExpress (NJ, USA). .. RBC lysis buffer (Cat#G2015), cell counting kit-8 assay kit (Cat#G4103), lactic acid assay kit (Cat#G4308), RIPA lysis buffer (Cat#G2002), IP lysis buffer (Cat# G2038), protease inhibitor cocktail (Cat#G2006), PMSF (Cat#G2008) and phosphatase inhibitor cocktail (Cat#G2007) were purchased from Servicebio (Wuhan, China).

    Article Title: PRC1 promotes immunosuppressive macrophages in sepsis via β-catenin/STAT3 signaling.
    Article Snippet: MSAB (Cat#S6901) and SKL2001 (Cat#S8320) were purchased from Selleck Chemicals (TX, USA). .. Protein A/G Magnetic Beads (Cat#HY-K0202), Mouse M-CSF (CatHY-P7085), human IFN-γ (Cat#HY-P7025) and mouse IFN-γ (Cat#HY-P7071) were purchased from MedChemExpress (NJ, USA). .. RBC lysis buffer (Cat#G2015), cell counting kit-8 assay kit (Cat#G4103), lactic acid assay kit (Cat#G4308), RIPA lysis buffer (Cat#G2002), IP lysis buffer (Cat# G2038), protease inhibitor cocktail (Cat#G2006), PMSF (Cat#G2008) and phosphatase inhibitor cocktail (Cat#G2007) were purchased from Servicebio (Wuhan, China).

    Incubation:

    Article Title: Rosavin regulates bone homeostasis through HDAC1-induced epigenetic regulation of EEF2.
    Article Snippet: Bioactive constituents from Rhodiola rosea L. show a myriad of pharmacological effects on diverse diseases.. Rosavin has been linked to reduced osteoclastogenesis, while its role in regulating osteogenesis remains unclear.. The present study investigated whether and how Rosavin alleviates ovariectomy (OVX)-induced osteoporosis (OP) in mice.

    Cell Culture:

    Article Title: IGSF9-targeted therapy inhibits the progression of acute myeloid leukemia
    Article Snippet: .. The remaining bone marrow cells were then cultured in the presence of 20 ng/mL mouse M-CSF (MedChemExpress, HY-P7085, RRID: AB_2923194) to differentiate into bone marrow-derived macrophages (BMDMs). .. After 3 days, the BMDMs were further polarized to induce M1 macrophages, 20 ng/mL IFN-γ (MedChemExpress, HY-P7025, RRID: AB_2923193) and 100 ng/mL LPS (MedChemExpress, HY-D1056, RRID: AB_2923195) were added for 24 hours; for M2 macrophage induction, 20 ng/mL IL-4 (PeproTech, 214-14-20UG, RRID: AB_2923196) and 20 ng/mL IL-13 (PeproTech, 210-13-10UG, RRID: AB_2923197) were administered for 48 hours.

    Recombinant:

    Article Title: α-Ketoglutarate plays an inflammatory inhibitory role by regulating scavenger receptor class a expression through N6-methyladenine methylation during sepsis.
    Article Snippet: Sepsis arises from an uncontrolled inflammatory response triggered by infection or stress, accompanied by alteration in cellular energy metabolism, and a strong correlation exists between these factors.. Alpha-ketoglutarate (α-KG), an intermediate product of the TCA cycle, has the potential to modulate the inflammatory response and is considered a crucial link between energy metabolism and inflammation.. The scavenger receptor (SR-A5), a significant pattern recognition receptor, assumes a vital function in anti-inflammatory reactions.

    Activity Assay:

    Article Title: α-Ketoglutarate plays an inflammatory inhibitory role by regulating scavenger receptor class a expression through N6-methyladenine methylation during sepsis.
    Article Snippet: Sepsis arises from an uncontrolled inflammatory response triggered by infection or stress, accompanied by alteration in cellular energy metabolism, and a strong correlation exists between these factors.. Alpha-ketoglutarate (α-KG), an intermediate product of the TCA cycle, has the potential to modulate the inflammatory response and is considered a crucial link between energy metabolism and inflammation.. The scavenger receptor (SR-A5), a significant pattern recognition receptor, assumes a vital function in anti-inflammatory reactions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: α-Ketoglutarate plays an inflammatory inhibitory role by regulating scavenger receptor class a expression through N6-methyladenine methylation during sepsis.
    Article Snippet: Sepsis arises from an uncontrolled inflammatory response triggered by infection or stress, accompanied by alteration in cellular energy metabolism, and a strong correlation exists between these factors.. Alpha-ketoglutarate (α-KG), an intermediate product of the TCA cycle, has the potential to modulate the inflammatory response and is considered a crucial link between energy metabolism and inflammation.. The scavenger receptor (SR-A5), a significant pattern recognition receptor, assumes a vital function in anti-inflammatory reactions.

    Methylation:

    Article Title: α-Ketoglutarate plays an inflammatory inhibitory role by regulating scavenger receptor class a expression through N6-methyladenine methylation during sepsis.
    Article Snippet: Sepsis arises from an uncontrolled inflammatory response triggered by infection or stress, accompanied by alteration in cellular energy metabolism, and a strong correlation exists between these factors.. Alpha-ketoglutarate (α-KG), an intermediate product of the TCA cycle, has the potential to modulate the inflammatory response and is considered a crucial link between energy metabolism and inflammation.. The scavenger receptor (SR-A5), a significant pattern recognition receptor, assumes a vital function in anti-inflammatory reactions.



    Similar Products

    98
    MedChemExpress macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/M-CSF%2C+Mouse/pmc13471083-120-17-23
    Average 98 stars, based on 1 article reviews
    macrophage colony - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    MedChemExpress recombinant macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Recombinant Macrophage Colony, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/M-CSF%2C+Mouse/pm42570377-143-19-25
    Average 98 stars, based on 1 article reviews
    recombinant macrophage colony - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    MedChemExpress murine recombinant m csf
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Murine Recombinant M Csf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/M-CSF%2C+Mouse/pmc13400354-321-18-21
    Average 98 stars, based on 1 article reviews
    murine recombinant m csf - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    MedChemExpress mouse m csf
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Mouse M Csf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/M-CSF%2C+Mouse/pmc13050104-39-30-32
    Average 98 stars, based on 1 article reviews
    mouse m csf - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    94
    MedChemExpress mouse mce cat
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Mouse Mce Cat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/M-CSF%2C+Mouse/pm42507550-262-202-203
    Average 94 stars, based on 1 article reviews
    mouse mce cat - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    98
    MedChemExpress m csf
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    M Csf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/M-CSF%2C+Mouse/pmc13400143-34-0-2
    Average 98 stars, based on 1 article reviews
    m csf - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    MedChemExpress macrophage colony stimulating factor
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Macrophage Colony Stimulating Factor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/M-CSF%2C+Mouse/pm42360493-44-51-58
    Average 98 stars, based on 1 article reviews
    macrophage colony stimulating factor - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    95
    Miltenyi Biotec macrophage colony
    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, <t>macrophage</t> <t>colony-stimulating</t> factor; BMDMs, bone marrow-derived macrophages.
    Macrophage Colony, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf/Mouse+M-CSF%2C+premium+grade/pm42263881-94-20-25
    Average 95 stars, based on 1 article reviews
    macrophage colony - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Journal: Frontiers in Immunology

    Article Title: APOE -associated lipid-handling macrophages in hepatocellular carcinoma: ligand–receptor communication and host Apoe -linked myeloid remodeling

    doi: 10.3389/fimmu.2026.1902143

    Figure Lengend Snippet: Apoe deficiency increases neutral lipid accumulation in lipid-loaded BMDMs (A) Schematic overview of BMDM isolation and BODIPY 493/503 staining. Bone marrow cells were isolated from WT and Apoe −/− mice and differentiated into BMDMs with M-CSF for 7 days. (B–D) Representative flow cytometry histograms and quantification of BODIPY 493/503 signal in WT and Apoe −/− BMDMs under untreated conditions (B) , after OA treatment (200 μM, 24 h) (C) , and after oxLDL treatment (50 μg/mL, 24 h) (D) . BODIPY 493/503 fluorescence was quantified as background-subtracted median fluorescence intensity (ΔMedian MFI). Data are shown as mean ± SEM. n = 3 biological replicates per group. P values were calculated using unpaired two-tailed Welch’s t-tests. ns, not significant; ** P < 0.01 ; *** P < 0.001 . OA, oleic acid; oxLDL, oxidized low-density lipoprotein; M-CSF, macrophage colony-stimulating factor; BMDMs, bone marrow-derived macrophages.

    Article Snippet: Bone marrow cells from WT and Apoe −/− mice were differentiated into bone marrow-derived macrophages (BMDMs) with macrophage colony-stimulating factor (M-CSF; 25 ng/mL; MedChemExpress, HY-P7085) for 7 days.

    Techniques: Isolation, Staining, Flow Cytometry, Fluorescence, Two Tailed Test, Derivative Assay